Prediction and Interpretation of the Volumetric Mass Transfer Coefficient in Bioreactors Using a No-Code Platform for Autonomous Machine Learning Model Selection

The volumetric mass transfer coefficient (kLa) governs the design, operation, and scale-up of aerobic bioprocesses, yet its dependence on reactor geometry, impeller design, operating conditions, and fluid properties limits prediction by empirical correlations. Machine learning (ML) improves accuracy but faces two barriers in bioprocess practice: selecting the best model among many candidates requires expertise, and small, highly multicollinear data make models chosen based on test error alone prone to overfitting. Using a browser-based, no-code platform, we trained 14 regression algorithms under an identical pipeline on a published kLa dataset, and introduced a composite objective, the generalization-penalized error (GPE), which is the test RMSE plus the absolute train–test RMSE gap. Minimizing GPE rather than test RMSE expanded the top statistically equivalent group to include not only …