Efficient Production of Shinorine and Mycosporine–Glycine–Alanine in Yarrowia lipolytica Using Natural and Engineered Nonribosomal Peptide Synthetases

High Resolution Image Download MS PowerPoint Slide Mycosporine-like amino acids (MAAs) are ultraviolet-absorbing metabolites with recognized photoprotective and pharmacological benefits, predominantly found in marine organisms. Due to the inefficiency of extraction from natural sources, microbial biosynthesis using heterologous hosts is an attractive alternative. Disubstituted MAAs, such as shinorine, porphyra-334, and mycosporine-2-glycine, are synthesized by conjugating serine, threonine, or glycine to mycosporine-glycine (MG), a reaction catalyzed by either a d -Ala- d -Ala ligase homolog (MysD) or a nonribosomal peptide synthetase (MysE). While MysD enzymes often yield diverse byproducts due to substrate promiscuity, MysE demonstrates higher substrate specificity; however, only a serine-specific MysE has been biochemically characterized. In this study, we enhanced shinorine productio…