Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand

Effective bone regeneration remains a significant challenge in surgical practice, particularly due to the limitations associated with autologous bone grafting, such as donor site morbidity and limited bone availability. This study investigated the potential of human bone-derived endothelial cells (b-ECs) in mediating bone regeneration, especially in conjunction with bone marrow-derived mesenchymal stem cells (bm-MSCs). It is demonstrated that b-ECs retain unique osteoinductive properties post-isolation, crucial for promoting bone formation in vivo. Utilizing ectopic and orthotopic xenograft models in immunodeficient mice, these findings revealed that the synergistic interaction of b-ECs and bm-MSCs induced rapid and substantial bone formation, highlighting the therapeutic potential of b-ECs in bone repair strategies. The distinct expression of KIT ligand (KITLG) in b-ECs emerged as a key…