Sulfur‐Doped Carbon Dots as a Highly Selective and Sensitive Fluorescent Probe for Copper Ion Detection in Biological Systems

Abstract Copper ions are regarded as a double‐edged sword since it plays a crucial role in biological functions but can also induce various severe diseases at imbalanced levels. However, conventional methods for quantifying copper ion (Cu 2+ ) levels often encounter complicated preparation procedures and interference from other substances. This study describes the one‐pot microwave synthesis of sulfur‐doped carbon dot (S_HICA) as a highly selective and sensitive fluorescent probe for Cu 2+ detection in biological systems. S_HICA demonstrates improved fluorescence properties such as increased intensity and a red‐shifted emission wavelength compared to carbon dots without sulfur (HICA), and S_HICA exhibits outstanding selectivity for Cu 2+ through a fluorescence quenching effect. In vitro and ex vivo experiments are performed to validate the fluorescence selectivity of S_HICA, revealing th…