Efficient myogenic commitment of human mesenchymal stem cells on biomimetic materials replicating myoblast topography

Recent developments in stem cell technologies have demonstrated human mesenchymal stem cells (hMSCs) as a possible cell source for cell-based therapies and regenerative medicine applications. Self-renewal and differentiation abilities of hMSCs have enabled hMSCs to be applied in regeneration of musculoskeletal tissue. hMSCs are able to myogenically differentiate via various approaches; however, the most efficient method has not been developed. Here, we describe the efficient commitment of hMSCs to the myogenic lineage on biomimetic substrates replicating myoblast topography. We have created a tissue culture platform that replicates the micro-and nanoscale topography of fully differentiated skeletal myoblasts. Using UV-assisted capillary force lithography, an optically transparent cellular model of fully differentiated myoblasts was developed using a UV curable poly(urethane acrylate) res…